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KMID : 0613820040140040702
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Journal of Life Science
2004 Volume.14 No. 4 p.702 ~ p.707
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Lactate Dehydrogenase Isozyme of Hypoxia Tropical Catfish (Pangasius polyuranodon, Hypostomus plecostomus)
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Cho Sung-Kyu
Yum Jung-Joo
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Abstract
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In native-polyacrylamide gel electrophoresis of Pangasius polyuranodon, the lactate dehydrogenase (EC 1.1.1.27, LDH) A4, A3B, A2B2, AB3 and B4 isozymes were expressed in various tissues. The LDH A4 and liver-specific C4 isozymes were expressed in the tissues of Hypostomus plecostomus. The bands of LDH in skeletal muscle, heart and eye tissues were not detected while one band was detected in kidney and liver, and four bands were detected in brain. The detected one band in liver was identified as alcohol dehydrogenase and an anodal band of skeletal muscle was identified as nothing dehydrogenase. The LDH in skeletal muscle, heart and eye might function as pyruvate reductase. The degree of inhibitions of LDH in skeletal muscle and heart of P. polyuranodon by 10 mM pyruvate were measured 57.6% and 73.8%, respectively. However, those of LDH in tissues of H. plecostomus were measured 52.7-61.8% so tissue specificity did not appear. Therefore, H. plecostomus might be more acclimated to hypoxic environment by anaerobic metabolism of LDH isozymes than P. polyuranodon.
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KEYWORD
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lactate dehydrogenase, isozymes, Pangasius polyuranodon, Hypostomus plecostomus
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